Gladiators 2000 is a spin-off television show of American Gladiators. It is hosted by Ryan Seacrest and Maria Sansone. Season 5 American Gladiators grand champion Peggy Odita served as head referee. It premiered on September 17, 1994 and ran until May 11, 1996. It was often partnered with its parent show in syndication, however some markets ran it independently. Like AG, the series was produced by Four Point Entertainment, and distributed by Samuel Goldwyn Television. In response to the popularity of NBC's 2008 revival of American Gladiators, the show was brought back in syndicated reruns for the 2008-2009 television season, being eligible for the FCC's "E/I" requirements.

In Gladiators 2000, two teams of two child competitors would team up with two Gladiators as coaches and compete by running through a series of events, and answering questions on various subjects along the way. While some props and sets were retained, the rules for each event were drastically altered.

A British version of this show was developed, known as Gladiators: Train 2 Win.